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基本信息

體類型 

植物雙元表達(dá)載體 

載體抗性 

Kan 

載體標(biāo)簽 

mfGFP5His 

篩選標(biāo)記 

潮霉素 

啟動子 

Lac,CaMV35s 

表達(dá)水平 

高拷貝 

載體大小 

10550bp 

克隆方法 

多克隆位點(diǎn),限制性內(nèi)切酶 

5' 測序引物 

M13 ReverseCAGGAAACAGCTATGAC 

3' 測序引物 

M13 ForwardTGTAAAACGACGGCCAGT 

備注:

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質(zhì)粒圖譜和多克隆位點(diǎn)信息

注意:

客戶在我公司選擇構(gòu)建載體服務(wù)項(xiàng)目時,請自行提供母源載體。

如要使用本公司保存的母源載體時,本公司不能保證所有母源載體完全符合客戶預(yù)期,也不承擔(dān)由此產(chǎn)生的任何責(zé)任。

 

 

載體序列

ORIGIN      

        1 catggtagat ctgactagta aaggagaaga acttttcact ggagttgtcc caattcttgt

       61 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga

      121 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc

      181 gtggccaaca cttgtcacta ctttctctta tggtgttcaa tgcttttcaa gatacccaga

      241 tcatatgaag cggcacgact tcttcaagag cgccatgcct gagggatacg tgcaggagag

      301 gaccatcttc ttcaaggacg acgggaacta caagacacgt gctgaagtca agtttgaggg

      361 agacaccctc gtcaacagga tcgagcttaa gggaatcgat ttcaaggagg acggaaacat

      421 cctcggccac aagttggaat acaactacaa ctcccacaac gtatacatca tggccgacaa

      481 gcaaaagaac ggcatcaaag ccaacttcaa gacccgccac aacatcgaag acggcggcgt

      541 gcaactcgct gatcattatc aacaaaatac tccaattggc gatggccctg tccttttacc

      601 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga

      661 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact

      721 atacaaagct agccaccacc accaccacca cgtgtgaatt ggtgaccagc tcgaatttcc

      781 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg

      841 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat

      901 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat

      961 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat

     1021 ctatgttact agatcgggaa ttaaactatc agtgtttgac aggatatatt ggcgggtaaa

     1081 cctaagagaa aagagcgttt attagaataa cggatattta aaagggcgtg aaaaggttta

     1141 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact

     1201 ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct

     1261 tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt

     1321 tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac

     1381 cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc

     1441 agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc

     1501 aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg

     1561 cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc

     1621 agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt

     1681 agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg

     1741 ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc

     1801 gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag

     1861 atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca

     1921 ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg

     1981 cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc

     2041 ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc

     2101 aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg

     2161 tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt

     2221 ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc

     2281 gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata

     2341 tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact

     2401 taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca

     2461 actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg

     2521 ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga

     2581 ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc

     2641 ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc

     2701 aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga

     2761 ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg

     2821 catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg

     2881 tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc

     2941 agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa

     3001 actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc

     3061 ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca

     3121 gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac

     3181 gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca

     3241 gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat

     3301 ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg

     3361 cggttggcca ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca ctggaacccc

     3421 caagcccgag gaatcggcgt gacggtcgca aaccatccgg cccggtacaa atcggcgcgg

     3481 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca

     3541 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag

     3601 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg

     3661 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca

     3721 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc

     3781 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg

     3841 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat

     3901 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac

     3961 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca

     4021 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc

     4081 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa

     4141 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag

     4201 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca

     4261 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt

     4321 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca

     4381 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg

     4441 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg

     4501 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc

     4561 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga

     4621 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag

     4681 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta

     4741 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc

     4801 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc

     4861 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc

     4921 gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc

     4981 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca

     5041 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt

     5101 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc

     5161 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac

     5221 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg

     5281 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa

     5341 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc

     5401 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc

     5461 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat

     5521 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc

     5581 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct

     5641 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg

     5701 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc

     5761 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga

     5821 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa

     5881 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta

     5941 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc

     6001 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg

     6061 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct aggtactaaa

     6121 acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta

     6181 agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc

     6241 agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac

     6301 ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa

     6361 gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg

     6421 gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat

     6481 ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg

     6541 agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat

     6601 cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc

     6661 catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca

     6721 tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt

     6781 ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt

     6841 ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca

     6901 ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc

     6961 taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca

     7021 gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc

     7081 gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc

     7141 taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat

     7201 agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc

     7261 cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc

     7321 tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt

     7381 aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat tcgggggatc

     7441 tggattttag tactggattt tggttttagg aattagaaat tttattgata gaagtatttt

     7501 acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata

     7561 ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa actcgagctt

     7621 gtcgatcgac agatccggtc ggcatctact ctatttcttt gccctcggac gagtgctggg

     7681 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct

     7741 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca

     7801 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg

     7861 gtcaagacca atgcggagca tatacgcccg gagtcgtggc gatcctgcaa gctccggatg

     7921 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa

     7981 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc

     8041 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg

     8101 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga

     8161 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc

     8221 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa

     8281 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atagcctccg cgaccggttg

     8341 tagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg

     8401 ggagatgcaa taggtcaggc tctcgctaaa ctccccaatg tcaagcactt ccggaatcgg

     8461 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca

     8521 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc

     8581 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt

     8641 ctcgacagac gtcgcggtga gttcaggctt tttcatatct cattgccccc cgggatctgc

     8701 gaaagctcga gagagataga tttgtagaga gagactggtg atttcagcgt gtcctctcca

     8761 aatgaaatga acttccttat atagaggaag gtcttgcgaa ggatagtggg attgtgcgtc

     8821 atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac

     8881 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc

     8941 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc

     9001 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag

     9061 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt

     9121 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttat cacatcaatc

     9181 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg

     9241 gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc

     9301 gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca

     9361 gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc

     9421 aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc

     9481 gtgctccacc atgttggcaa gctgctctag ccaatacgca aaccgcctct ccccgcgcgt

     9541 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag

     9601 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg

     9661 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc

     9721 tatgaccatg attacgaatt cgagctcggt acccggggat cctctagagt cgacctgcag

     9781 gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt

     9841 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga

     9901 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag

     9961 cttgagcttg gatcagattg tcgtttcccg ccttcagttt agcttcatgg agtcaaagat

    10021 tcaaatagag gacctaacag aactcgccgt aaagactggc gaacagttca tacagagtct

    10081 cttacgactc aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acacacttgt

    10141 ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg agacttttca

    10201 acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct gtcactttat

    10261 tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa

    10321 ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag

    10381 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga

    10441 tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc

    10501 tatataagga agttcatttc atttggagag aacacggggg actcttgac