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載體描述

   TRV based VIGS vectors described in Liu et al. 2002). TRV cDNA clones were placed in between duplicated CaMV 35S promoter (2 x 35S) and nopalinesynthase terminator (NOSt) in a T-DNA vector. RdRp, RNA dependent RNA polymerase; 16K, 16 kDa cysteine rich protein; MP, movement protein; CP, coatprotein; LB and RB, left and right borders of T-DNA; Rz, self-cleaving ribozyme; MCS, multiple cloning sites.

   pTRV1. Tobacco rattle virus (TRV) based cDNA clone was placed between the duplicated CaMV 35S promoter and the nopaline synthase terminator in a T-DNA vector.

載體序列

pTRV1序列，不是全長(zhǎng)。

         1 ataaaacatt tcaatccttt gaacgcggta gaacgtgcta attggatttt ggtgagaacg

        61 cggtagaacg tacttatcac ctacagtttt attttgtttt tctttttggt ttaatctatc

      121 cagcttagta ccgagtgggg gaaagtgact ggtgtgccta aaaccttttc tttgatactt

      181 tgtaaaaata catacagata caatggcgaa cggtaacttc aagttgtctc aattgctcaa

      241 tgtggacgag atgtctgctg agcagaggag tcatttcttt gacttgatgc tgactaaacc

      301 tgattgtgag atcgggcaaa tgatgcaaag agttgttgtt gataaagtcg atgacatgat

      361 tagagaaaga aagactaaag atccagtgat tgttcatgaa gttctttctc agaaggaaca

      421 gaacaagttg atggaaattt atcctgaatt caatatcgtg tttaaagacg acaaaaacat

      481 ggttcatggg tttgcggctg ctgagcgaaa actacaagct ttattgcttt tagatagagt

      541 tcctgctctg caagaggtgg atgacatcgg tggtcaatgg tcgttttggg taactagagg

      601 tgagaaaagg attcattcct gttgtccaaa tctagatatt cgggatgatc agagagaaat

      661 ttctcgacag atatttctta ctgctattgg tgatcaagct agaagtggta agagacagat

      721 gtcggagaat gagctgtgga tgtatgacca atttcgtgaa aatattgctg cgcctaacgc

      781 ggttaggtgc aataatacat atcagggttg tacatgtagg ggtttttctg atggtaagaa

      841 gaaaggcgcg cagtatgcga tagctcttca cagcctgtat gacttcaagt tgaaagactt

      901 gatggctact atggttgaga agaaaactaa agtggttcat gctgctatgc tttttgctcc

      961 tgaaagtatg ttagtggacg aaggtccatt accttctgtt gacggttact acatgaagaa

     1021 gaacgggaag atctatttcg gttttgagaa agatccttcc ttttcttaca ttcatgactg

     1081 ggaagagtac aagaagtatc tactggggaa gccagtgagt taccaaggga atgtgttcta

     1141 cttcgaaccg tggcaggtga gaggagacac aatgcttttt tcgatctaca ggatagctgg

     1201 agttccgagg aggtctctat catcgcaaga gtactaccga agaatatata tcagtagatg

     1261 ggaaaacatg gttgttgtcc caattttcga tctggtcgaa tcaacgcgag agttggtcaa

     1321 gaaagacctg tttgtagaga aacaattcat ggacaagtgt ttggattaca tagctaggtt

     1381 atctgaccag cagctgacca taagcaatgt taaatcatac ttgagttcaa ataattgggt

     1441 cttattcata aacggggcgg ccgtgaagaa caagcaaagt gtagattctc gagatttaca

     1501 gttgttggct caaactttgc tagtgaagga acaagtggcg agacctgtca tgagggagtt

     1561 gcgtgaagca attctgactg agacgaaacc tatcacgtca ttgactgatg tgctgggttt

     1621 aatatcaaga aaactgtgga agcagtttgc taacaagatc gcagtcggcg gattcgttgg

     1681 catggttggt actctaattg gattctatcc aaagaaggta ctaacctggg cgaaggacac

     1741 accaaatggt ccagaactat gttacgagaa ctcgcacaaa accaaggtga tagtatttct

     1801 gagtgttgtg tatgccattg gaggaatcac gcttatgcgt cgagacatcc gagatggact

     1861 ggtgaaaaaa ctatgtgata tgtttgatat caaacggggg gcccatgtct tagacgttga

     1921 gaatccgtgc cgctattatg aaatcaacga tttctttagc agtctgtatt cggcatctga

     1981 gtccggtgag accgttttac cagatttatc cgaggtaaaa gccaagtctg ataagctatt

     2041 gcagcagaag aaagaaatcg ctgacgagtt tctaagtgca aaattctcta actattctgg

     2101 cagttcggtg agaacttctc caccatcggt ggtcggttca tctcgaagcg gactgggtct

     2161 gttgttggaa gacagtaacg tgctgaccca agctagagtt ggagtttcaa gaaaggtaga

     2221 cgatgaggag atcatggagc agtttctgag tggtcttatt gacactgaag cagaaattga

     2281 cgaggttgtt ccagcctttt cagctgaatg tgaaagaggg gaaacaagcg gtacaaaggt

     2341 gttgtgtaaa cctttaacgc caccaggatt tgagaacgtg ttgccagctg tcaaaccttt

     2401 ggtcagcaaa ggaaaaacgg tcaaacgtgt cgattacttc caagtgatgg gaggtgagag

     2461 attaccaaaa aggccggttg tcagtggaga cgattctgtg gacgctagaa gagagtttct

     2521 gtactactta gatgcggaga gagtcgctca aaatgatgaa attatgtctc tgtatcgtga

     2581 ctattcgaga ggagttattc gaactggagg tcagaattac ccgcacggac tgggagtgtg

     2641 ggatgtggag atgaagaact ggtgcatacg tccagtggtc actgaacatg cttatgtgtt

     2701 ccaaccagac aaacgtatgg atgattggtc gggatactta gaagtggctg tttgggaacg

     2761 aggtatgttg gtcaacgact tcgcggtcga aaggatgagt gattatgtca tagtttgcga

     2821 tcagacgtat ctttgcaata acaggttgat cttggacaat ttaagtgccc tggatctagg

     2881 accagttaac tgttcttttg aattagttga cggtgtacct ggttgtggta agtcgacaat

     2941 gattgtcaac tcagctaatc cttgtgtcga tgtggttctc tctactggga gagcagcaac

     3001 cgacgacttg atcgagagat tcgcgagcaa aggttttcca tgcaaattga aaaggagagt

     3061 gaagacggtt gattcttttt tgatgcattg tgttgatggt tctttaaccg gagacgtgtt

     3121 gcatttcgat gaagctctca tggcccatgc tggtatggtg tacttttgcg ctcagatagc

     3181 tggtgctaaa cgatgtatct gtcaaggaga tcagaatcaa atttctttca agcctagggt

     3241 atctcaagtt gatttgaggt tttctagtct ggtcggaaag tttgacattg ttacagaaaa

     3301 aagagaaact tacagaagtc cagcagatgt ggctgccgta ttgaacaagt actatactgg

     3361 agatgtcaga acacataacg cgactgctaa ttcgatgacg gtgaggaaga ttgtgtctaa

     3421 agaacaggtt tctttgaagc ctggtgctca gtacataact ttccttcagt ctgagaagaa

     3481 ggagttggta aatttgttgg cattgaggaa agtggcagct aaagtgagta cagtacacga

     3541 gtcgcaagga gagacattca aagatgtagt cctagtcagg acgaaaccta cggatgactc

     3601 aatcgctaga ggtcgggagt acttaatcgt ggcgttgtcg cgtcacacac aatcacttgt

     3661 gtatgaaact gtgaaagagg acgatgtaag caaagagatc agggaaagtg ccgcgcttac

     3721 gaaggcggct ttggcaagat tttttgttac tgagaccgtc ttatgacggt ttcggtctag

     3781 gtttgatgtc tttagacatc atgaagggcc ttgcgccgtt ccagattcag gtacgattac

     3841 ggacttggag atgtggtacg acgctttgtt tccgggaaat tcgttaagag actcaagcct

     3901 agacgggtat ttggtggcaa cgactgattg caatttgcga ttagacaatg ttacgatcaa

     3961 aagtggaaac tggaaagaca agtttgctga aaaagaaacg tttctgaaac cggttattcg

     4021 tactgctatg cctgacaaaa ggaagactac tcagttggag agtttgttag cattgcagaa

     4081 aaggaaccaa gcggcacccg atctacaaga aaatgtgcac gcaacagttc taatcgaaga

     4141 gacgatgaag aagttgaaat ctgttgtcta cgatgtggga aaaattcggg ctgatcctat

     4201 tgtcaataga gctcaaatgg agagatggtg gagaaatcaa agcacagcgg tacaggctaa

     4261 ggtagtagca gatgtgagag agttacatga aatagactat tcgtcttaca tgtatatgat

     4321 caaatctgac gtgaaaccta agactgattt aacaccgcaa tttgaatact cagctctaca

     4381 gactgttgtg tatcacgaga agttgatcaa ctcgttgttc ggtccaattt tcaaagaaat

     4441 taatgaacgc aagttggatg ctatgcaacc acattttgtg ttcaacacga gaatgacatc

     4501 gagtgattta aacgatcgag tgaagttctt aaatacggaa gcggcttacg actttgttga

     4561 gatagacatg tctaaattcg acaagtcggc aaatcgcttc catttacaac tgcagctgga

     4621 gatttacagg ttatttgggc tagatgagtg ggcggccttc ctttgggagg tgtcgcacac

     4681 tcaaactact gtgagagata ttcaaaatgg tatgatggcg catatttggt accaacaaaa

     4741 gagtggagat gctgatactt ataatgcaaa ttcagataga acactgtgtg cactcttgtc

     4801 tgaattacca ttggagaaag cagtcatggt tacatatgga ggagatgact cactgattgc

     4861 gtttcctaga ggaacgcagt ttgttgatcc gtgtccaaag ttggctacta agtggaattt

     4921 cgagtgcaag atttttaagt acgatgtccc aatgttttgt gggaagttct tgcttaagac

     4981 gtcatcgtgt tacgagttcg tgccagatcc ggtaaaagtt ctgacgaagt tggggaaaaa

     5041 gagtataaag gatgtgcaac atttagccga gatctacatc tcgctgaatg attccaatag

     5101 agctcttggg aactacatgg tggtatccaa actgtccgag tctgtttcag accggtattt

     5161 gtacaaaggt gattctgttc atgcgctttg tgcgctatgg aagcatatta agagttttac

     5221 agctctgtgt acattattcc gagacgaaaa cgataaggaa ttgaacccgg ctaaggttga

     5281 ttggaagaag gcacagagag ctgtgtcaaa cttttacgac tggtaatatg gaagacaagt

     5341 cattggtcac cttgaagaag aagactttcg aagtctcaaa attctcaaat ctaggggcca

     5401 ttgaattgtt tgtggacggt aggaggaaga gaccgaagta ttttcacaga agaagagaaa

     5461 ctgtcctaaa tcatgttggt gggaagaaga gtgaacacaa gttagacgtt tttgaccaaa

     5521 gggattacaa aatgattaaa tcttacgcgt ttctaaagat agtaggtgta caactagttg

     5581 taacatcaca tctacctgca gatacgcctg ggttcattca aatcgatctg ttggattcga

     5641 gacttactga gaaaagaaag agaggaaaga ctattcagag attcaaagct cgagcttgcg

     5701 ataactgttc agttgcgcag tacaaggttg aatacagtat ttccacacag gagaacgtac

     5761 ttgatgtctg gaaggtgggt tgtatttctg agggcgttcc ggtctgtgac ggtacatacc

     5821 ctttcagtat cgaagtgtcg ctaatatggg ttgctactga ttcgactagg cgcctcaatg

     5881 tggaagaact gaacagttcg gattacattg aaggcgattt taccgatcaa gaggttttcg

     5941 gtgagttcat gtctttgaaa caagtggaga tgaagacgat tgaggcgaag tacgatggtc

     6001 cttacagacc agctactact agacctaagt cattattgtc aagtgaagat gttaagagag

     6061 cgtctaataa gaaaaactcg tcttaatgca taaagaaatt tattgtcaat atgacgtgtg

     6121 tactcaaggg ttgtgtgaat gaagtcactg ttcttggtca cgagacgtgt agtatcggtc

     6181 atgctaacaa attgcgaaag caagttgctg acatggttgg tgtcacacgt aggtgtgcgg

     6241 aaaataattg tggatggttt gtctgtgttg ttatcaatga ttttactttt gatgtgtata

     6301 attgttgtgg ccgtagtcac cttgaaaagt gtcgtaaacg tgttgaaaca agaaatcgag

     6361 aaatttggaa acaaattcga cgaaatcaag ctgaaaacat gtctgcgaca gctaaaaagt

     6421 ctcataattc gaagacctct aagaagaaat tcaaagagga cagagaattt gggacaccaa

     6481 aaagattttt aagagatgat gttcctttcg ggattgatcg tttgtttgct ttttgatttt

     6541 attttatatt gttatctgtt tctgtgtata gactgtttga gattggcgct tggccgactc

     6601 attgtcttac cataggggaa cggactttgt ttgtgttgtt attttatttg tattttatta

     6661 aaattctcaa tgatctgaaa aggcctcgag gctaagagat tattgggggg tgagtaagta

     6721 cttttaaagt gatgatggtt acaaaggcaa aaggggtaaa acccctcgcc tacgtaagcg

     6781 ttattacgcc c

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