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基本信息

質(zhì)粒圖譜和多克隆位點信息

注意：

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載體描述

植物過表達載體，帶GFP報告基因，pGreenII-62-SK載體改造而來，融合表達GFP蛋白。

載體序列

LOCUS       Exported                4055 bp ds-DNA     circular SYN 27-MAR-2019

DEFINITION  .

ACCESSION   .

VERSION     .

KEYWORDS    pGreenII 62-SK-GFP

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 4055)

  AUTHORS   BT-Lab

  TITLE     Direct Submission

  JOURNAL   Exported Wednesday, March 27, 2019 from SnapGene 3.2.1

FEATURES             Location/Qualifiers

     source          1..4055

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     misc_feature    542..564

                     /note="LB T-DNA repeat"

                     /note="left border repeat from nopaline C58 T-DNA

                     (truncated)"

                     /note="color: #ffe4c4"

     promoter        638..982

                     /note="CaMV 35S promoter"

                     /note="strong constitutive promoter from cauliflower mosaic

                     virus"

                     /note="color: #ffffff; direction: RIGHT"

     misc_feature    992..1085

                     /note="MCS"

                     /note="pBluescript multiple cloning site"

                     /note="color: #99ccff"

     primer_bind     1016..1032

                     /note="SK primer"

                     /note="common sequencing primer, one of multiple similar

                     variants"

                     /note="color: #a020f0; direction: RIGHT"

     primer_bind     complement(1066..1082)

                     /note="KS primer"

                     /note="common sequencing primer, one of multiple similar

                     variants"

                     /note="color: #a020f0; direction: LEFT"

     misc_feature    1085..1801

                     /note="GFP"

                     /note="color: #ccffcc; direction: RIGHT"

     polyA_signal    1861..2037

                     /note="CaMV poly(A) signal"

                     /note="cauliflower mosaic virus polyadenylation signal"

                     /note="color: #a6acb3"

     misc_feature    2047..2071

                     /note="RB T-DNA repeat"

                     /note="right border repeat from nopaline C58 T-DNA"

                     /note="color: #ffe4c4"

     rep_origin      complement(2162..2750)

                     /direction=LEFT

                     /note="ori"

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

                     replication"

                     /note="color: #ffff00"

     CDS             complement(2921..3736)

                     /codon_start=1

                     /gene="aph(3')-Ia"

                     /product="aminoglycoside phosphotransferase"

                     /note="KanR"

                     /note="confers resistance to kanamycin in bacteria or G418

                     (Geneticin(R)) in eukaryotes"

                     /note="color: #ccffcc"

                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG

                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK

                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA

                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI

                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"

     rep_origin      4027..407

                     /note="pSa ori"

                     /note="origin of replication from bacterial plasmid pSa"

                     /note="color: #ffff00"

ORIGIN

        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc

       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg

      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg

      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc

      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat

      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac

      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc

      421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa

      481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc

      541 ttggcaggat atattgtggt gtaacgttat cagcttggta cgtacccccc tactccaaaa

      601 atgtcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa

      661 tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag

      721 tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc

      781 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg

      841 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgac atctccactg

      901 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa

      961 gtcatttcat ttggagagga cagcccaagc tgagctccac cgcggtggcg gccgctctag

     1021 aactagtgga tcccccgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct

     1081 cgagatgggc aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga

     1141 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata

     1201 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac

     1261 acttgtcact actttcactt atggtgttca atgcttttca agatacccag atcatatgaa

     1321 gcggcacgac ttcttcaaga gcgccatgcc tgagggatac gtgcaggaga ggaccatctc

     1381 tttcaaggac gacgggaact acaagacacg tgctgaagtc aagtttgagg gagacaccct

     1441 cgtcaacagg atcgagctta agggaatcga tttcaaggag gacggaaaca tcctcggcca

     1501 caagttggaa tacaactaca actcccacaa cgtatacatc acggcagaca aacaaaagaa

     1561 tggaatcaaa gctaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc

     1621 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca

     1681 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt

     1741 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata

     1801 aggtaccaat tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat

     1861 tttctccata aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg

     1921 tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac

     1981 ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatccac

     2041 tagccttgac aggatatatt ggcgggtaaa ctaagtcgct gtatgtgttt gtttgagatc

     2101 tcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt

     2161 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt

     2221 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc

     2281 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa

     2341 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct

     2401 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta

     2461 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg

     2521 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc

     2581 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta

     2641 ccttcggaag aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg

     2701 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt

     2761 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg

     2821 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta

     2881 aatcaatcta aagtatatat gtgtaacatt ggtctagtga ttagaaaaac tcatcgagca

     2941 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc

     3001 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt

     3061 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa

     3121 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca

     3181 aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa

     3241 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata

     3301 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca

     3361 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg

     3421 ctgttttccc tgggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat

     3481 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg

     3541 taacaacatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct

     3601 tcccatacaa tcggtagatt gtcgcacctg attgcccgac attatcgcga gcccatttat

     3661 acccatataa atcagcatcc atgttggaat ttaatcgcgg ccttgagcaa gacgtttccc

     3721 gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg

     3781 ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaacgt

     3841 ggctttgttg aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg

     3901 acaacgcaga ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg tccacctaca

     3961 acaaagctct catcaaccgt ggctccctca ctttctggct ggatgatggg gcgattcagg

     4021 cgatccccat ccaacagccc gccgtcgagc gggct

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