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基本信息
體類型: 植物表達(dá)載體 載體抗性: 卡那霉素 載體標(biāo)簽: C-GFP 篩選標(biāo)記: / 啟動(dòng)子: CaMV 35S 表達(dá)水平: 高 載體大小: 4055bp 克隆方法: 多克隆位點(diǎn),限制性內(nèi)切酶 5' 測(cè)序引物: 35S:5’-GACGCACAATCCCACTATCC-3’ 3' 測(cè)序引物: KS primer: 5’-CGAGGTCGACGGTATCG-3’ 組成型: 組成型 備注 pGreenII-62-SK改造而來(lái),插入融合表達(dá)GFP蛋白,由轉(zhuǎn)導(dǎo)生物公司改造,編號(hào)2181.
質(zhì)粒圖譜和多克隆位點(diǎn)信息
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載體描述
植物過(guò)表達(dá)載體,帶GFP報(bào)告基因,pGreenII-62-SK載體改造而來(lái),融合表達(dá)GFP蛋白。
載體序列
LOCUS Exported 4055 bp ds-DNA circular SYN 27-MAR-2019
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS pGreenII 62-SK-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4055)
AUTHORS BT-Lab
TITLE Direct Submission
JOURNAL Exported Wednesday, March 27, 2019 from SnapGene 3.2.1
FEATURES Location/Qualifiers
source 1..4055
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 542..564
/note="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA
(truncated)"
/note="color: #ffe4c4"
promoter 638..982
/note="CaMV 35S promoter"
/note="strong constitutive promoter from cauliflower mosaic
virus"
/note="color: #ffffff; direction: RIGHT"
misc_feature 992..1085
/note="MCS"
/note="pBluescript multiple cloning site"
/note="color: #99ccff"
primer_bind 1016..1032
/note="SK primer"
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0; direction: RIGHT"
primer_bind complement(1066..1082)
/note="KS primer"
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0; direction: LEFT"
misc_feature 1085..1801
/note="GFP"
/note="color: #ccffcc; direction: RIGHT"
polyA_signal 1861..2037
/note="CaMV poly(A) signal"
/note="cauliflower mosaic virus polyadenylation signal"
/note="color: #a6acb3"
misc_feature 2047..2071
/note="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
/note="color: #ffe4c4"
rep_origin complement(2162..2750)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
CDS complement(2921..3736)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/note="color: #ccffcc"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 4027..407
/note="pSa ori"
/note="origin of replication from bacterial plasmid pSa"
/note="color: #ffff00"
ORIGIN
1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc
421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc
541 ttggcaggat atattgtggt gtaacgttat cagcttggta cgtacccccc tactccaaaa
601 atgtcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa
661 tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag
721 tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc
781 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
841 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgac atctccactg
901 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
961 gtcatttcat ttggagagga cagcccaagc tgagctccac cgcggtggcg gccgctctag
1021 aactagtgga tcccccgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct
1081 cgagatgggc aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
1141 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
1201 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
1261 acttgtcact actttcactt atggtgttca atgcttttca agatacccag atcatatgaa
1321 gcggcacgac ttcttcaaga gcgccatgcc tgagggatac gtgcaggaga ggaccatctc
1381 tttcaaggac gacgggaact acaagacacg tgctgaagtc aagtttgagg gagacaccct
1441 cgtcaacagg atcgagctta agggaatcga tttcaaggag gacggaaaca tcctcggcca
1501 caagttggaa tacaactaca actcccacaa cgtatacatc acggcagaca aacaaaagaa
1561 tggaatcaaa gctaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
1621 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
1681 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
1741 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata
1801 aggtaccaat tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat
1861 tttctccata aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg
1921 tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac
1981 ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatccac
2041 tagccttgac aggatatatt ggcgggtaaa ctaagtcgct gtatgtgttt gtttgagatc
2101 tcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
2161 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
2221 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
2281 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
2341 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
2401 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
2461 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
2521 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
2581 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
2641 ccttcggaag aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
2701 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
2761 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
2821 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
2881 aatcaatcta aagtatatat gtgtaacatt ggtctagtga ttagaaaaac tcatcgagca
2941 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
3001 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
3061 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
3121 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
3181 aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
3241 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata
3301 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
3361 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
3421 ctgttttccc tgggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
3481 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
3541 taacaacatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
3601 tcccatacaa tcggtagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
3661 acccatataa atcagcatcc atgttggaat ttaatcgcgg ccttgagcaa gacgtttccc
3721 gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg
3781 ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaacgt
3841 ggctttgttg aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg
3901 acaacgcaga ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg tccacctaca
3961 acaaagctct catcaaccgt ggctccctca ctttctggct ggatgatggg gcgattcagg
4021 cgatccccat ccaacagccc gccgtcgagc gggct
//