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基本信息

載體類型： 

大腸桿菌蛋白雙表達(dá)載體，有兩個(gè)多克隆位點(diǎn)（雙啟動(dòng)子）      

載體抗性： 

Chl 

載體標(biāo)簽： 

N-His, C-S 

啟動(dòng)子： 

T7；lac 

篩選標(biāo)記：

Puro 

表達(dá)水平: 

低拷貝 

載體大小: 

4008bp 

克隆方法: 

多克隆位點(diǎn)，限制性內(nèi)切酶 

5' 測(cè)序引物: 

DUETF1: 5'-TGGTGTCCGGGATCTCGA-3'   

DUETF2: 5'-TTGTACACGGCCGCATAATC-3'   

3' 測(cè)序引物： 

DUETR1: 5'-GATTATGCGGCCGTGTACAA-3'   

T7 ter: 5'-TGCTAGTTATTGCTCAGCGG-3'    

備注：

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質(zhì)粒圖譜和多克隆位點(diǎn)信息

注意：

客戶在我公司選擇構(gòu)建載體服務(wù)項(xiàng)目時(shí)，請(qǐng)自行提供母源載體。

如要使用本公司保存的母源載體時(shí)，本公司不能保證所有母源載體完全符合客戶預(yù)期，也不承擔(dān)由此產(chǎn)生的任何責(zé)任。

載體描述

pACYCDue-1 is designed for the co-expression of two target genes. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7 promoter/lac operator and ribosome binding site (rbs). The vector also carries the P15A replicon, lacI gene and chloramphenicol resistance gene. This vector can be used in combination with pACYCDuet?-1 (Cat. No. 71146-3) in an appropriate host strain for the coexpression of up to 4 target genes. Genes inserted into MCS1 can be sequenced using the ACYCDuetUP1 Primer (Cat. No. 71178-3) and DuetDOWN1 Primer (Cat. No. 71179- 3). Genes inserted into MCS2 can be sequenced using the DuetUP2 Primer (Cat. No. 71180-3) and T7 Terminator Primer (Cat. No. 69337-3).

載體序列

ORIGIN

        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag

       61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag

      121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag

      181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt

      241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata

      301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt

      361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg

      421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg

      481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg

      541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt

      601 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat

      661 ccgcttatta tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct

      721 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt

      781 ctgccgacat ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc

      841 accttgtcgc cttgcgtata atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc

      901 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa

      961 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca

     1021 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat

     1081 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc

     1141 accagctcac cgtctttcat tgccatacgg aactccggat gagcattcat caggcgggca

     1201 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag

     1261 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc

     1321 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt

     1381 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt

     1441 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat

     1501 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat

     1561 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct

     1621 gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc

     1681 tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc

     1741 cggacatcag cgctagcgga gtgtatactg gcttactatg ttggcactga tgagggtgtc

     1801 agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt

     1861 gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc

     1921 ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc aggaagatac

     1981 ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga

     2041 caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag

     2101 ataccaggcg tttcccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt

     2161 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc

     2221 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg

     2281 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc

     2341 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt

     2401 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt

     2461 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt

     2521 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc

     2581 agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca

     2641 gccccatacg atataagttg taattctcat gttagtcatg ccccgcgccc accggaagga

     2701 gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag

     2761 ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg

     2821 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca

     2881 gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc

     2941 cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt

     3001 tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta

     3061 ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg

     3121 ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca

     3181 tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa

     3241 tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac

     3301 ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc

     3361 ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga

     3421 catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt

     3481 catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg

     3541 ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca

     3601 gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac

     3661 tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt

     3721 tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa

     3781 cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg

     3841 cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc

     3901 gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc

     3961 tctcccttat gcgactcctg cattaggaaa ttaatacgac tcactata

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