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基本信息

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載體描述

pCAMBIA1300-35S-3XFlag在pCAMBIA1300-35S載體的基礎(chǔ)上改造而來，植物融合表達(dá)載體。

載體序列

ORIGIN

        1 gaattcggtc cccagattag ccttttcaat ttcagaaaga atgctaaccc acagatggtt

       61 agagaggctt acgcagcagg tctcatcaag acgatctacc cgagcaataa tctccaggaa

      121 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc

      181 aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt

      241 caaggcttgc ttcacaaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt

      301 cccactgaat caaaggccat ggagtcaaag attcaaatag aggacctaac agaactcgcc

      361 gtaaagactg gcgaacagtt catacagagt ctcttacgac tcaatgacaa gaagaaaatc

      421 ttcgtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc

      481 tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc

      541 ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc

      601 tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac

      661 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca

      721 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca

      781 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag

      841 agaacacggg ggacgagctc ggtacccggg gatcctctag agtcgacatg gactacaaag

      901 accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtaac

      961 tgcagagctt tcgttcgtat catcggtttc gacaacgttc gtcaagttca atgcatcagt

     1021 ttcattgcgc acacaccaga atcctactga gttcgagtat tatggcattg ggaaacatgt

     1081 ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg ttttcgctat

     1141 cgaactgtga aatggaaatg gatggagaag agttaatgaa tgatatggtc cttttgttca

     1201 ttctcaaatt aatattattt gttttttctc ttatttgttg tgtgttgaat ttgaaaatat

     1261 aagagatatg caaacatttt gttttgagta aaaatgtgtc aaatcgtggc ctctaatgac

     1321 cgaagttaat atgaggagta aaacacttgt agttgtacca ttatgcttat tcactaggca

     1381 acaaatatat tttcagacct agaaaagctg caaatgttac tgaatacaag tatgtcctct

     1441 tgtgttttag acatttatga actttccttt atgtaatttt ccagaatcct tgtcagattc

     1501 taatcattgc tttataatta tagttatact catggatttg tagttgagta tgaaaatatt

     1561 ttttaatgca ttttatgact tgccaattga ttgacaacat gcatcaagct tggcactggc

     1621 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc

     1681 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc

     1741 ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg atcagattgt

     1801 cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc gggtaaacct

     1861 aagagaaaag agcgtttatt agaataacgg atatttaaaa gggcgtgaaa aggtttatcc

     1921 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg

     1981 atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct

     2041 gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc

     2101 tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg

     2161 agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc

     2221 accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag

     2281 ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt

     2341 gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc

     2401 acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc

     2461 ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc

     2521 gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag

     2581 gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc

     2641 gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg

     2701 cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc

     2761 accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg

     2821 gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg

     2881 acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt

     2941 tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg

     3001 atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc

     3061 taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga

     3121 tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa

     3181 ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact

     3241 cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc

     3301 ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg

     3361 cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc

     3421 ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag

     3481 cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt

     3541 cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat

     3601 cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat

     3661 cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga

     3721 acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact

     3781 catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc

     3841 cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc

     3901 atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg

     3961 gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag

     4021 taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga

     4081 aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg

     4141 ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa

     4201 gcccgaggaa tcggcgtgac ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc

     4261 tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg

     4321 aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat

     4381 cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc

     4441 aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca

     4501 tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct

     4561 acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt

     4621 gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc

     4681 gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca

     4741 agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc

     4801 ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg

     4861 tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg

     4921 ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag

     4981 gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg

     5041 gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt

     5101 tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg

     5161 tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc

     5221 aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg

     5281 gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa

     5341 aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc

     5401 ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga

     5461 ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa

     5521 ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc

     5581 aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta

     5641 tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg

     5701 gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg

     5761 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct

     5821 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc

     5881 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta

     5941 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc

     6001 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact

     6061 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac

     6121 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa

     6181 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg

     6241 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa

     6301 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc

     6361 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac

     6421 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac

     6481 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg

     6541 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt

     6601 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga

     6661 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct

     6721 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga

     6781 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg

     6841 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcattctagg tactaaaaca

     6901 attcatccag taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt

     6961 caaaaaatag ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga

     7021 aggcaatgtc ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta

     7081 ctttgccatc tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt

     7141 cctcttcggg cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag

     7201 tgtcttcttc ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca

     7261 attcggctaa gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt

     7321 gaaagagcct gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt

     7381 catactcttc cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat

     7441 catgccgttc aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca

     7501 tgtccttttc ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta

     7561 aatataggtt ttcattttct cccaccagct tatatacctt agcaggagac attccttccg

     7621 tatcttttac gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt

     7681 tagccattta ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa

     7741 ttataacaag acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa

     7801 aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat

     7861 tttgaaaccg cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac

     7921 cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc

     7981 atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg

     8041 actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt

     8101 tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat

     8161 aacacattgc ggacgttttt aatgtactga attaacgccg aattaattcg ggggatctgg

     8221 attttagtac tggattttgg ttttaggaat tagaaatttt attgatagaa gtattttaca

     8281 aatacaaata catactaagg gtttcttata tgctcaacac atgagcgaaa ccctatagga

     8341 accctaattc ccttatctgg gaactactca cacattatta tggagaaact cgagcttgtc

     8401 gatcgacaga tccggtcggc atctactcta tttctttgcc ctcggacgag tgctggggcg

     8461 tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct

     8521 gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg

     8581 accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc

     8641 aagaccaatg cggagcatat acgcccggag tcgtggcgat cctgcaagct ccggatgcct

     8701 ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat

     8761 gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt

     8821 tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg

     8881 gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc

     8941 actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca

     9001 tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc

     9061 gctcgtctgg ctaagatcgg ccgcagcgat cgcatccata gcctccgcga ccggttgtag

     9121 aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg cacggcggga

     9181 gatgcaatag gtcaggctct cgctaaactc cccaatgtca agcacttccg gaatcgggag

     9241 cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct

     9301 atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc

     9361 gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc

     9421 gacagacgtc gcggtgagtt caggcttttt catatctcat tgccccccgg gatctgcgaa

     9481 agctcgagag agatagattt gtagagagag actggtgatt tcagcgtgtc ctctccaaat

     9541 gaaatgaact tccttatata gaggaaggtc ttgcgaagga tagtgggatt gtgcgtcatc

     9601 ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc

     9661 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga

     9721 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc

     9781 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt

     9841 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc

     9901 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttatcac atcaatccac

     9961 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg

    10021 tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca

    10081 atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat

    10141 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat

    10201 agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg

    10261 ctccaccatg ttggcaagct gctctagcca atacgcaaac cgcctctccc cgcgcgttgg

    10321 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc

    10381 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt

    10441 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat

    10501 gacatgatta c

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