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基本信息

質(zhì)粒圖譜和多克隆位點(diǎn)信息

注意：

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載體描述

pAc5.1 - EGFP用于在昆蟲(chóng)細(xì)胞中表達(dá)帶有EGFP標(biāo)簽的蛋白，可以與篩選載體pCoHygro或者pCoBlast共轉(zhuǎn)染，建立穩(wěn)表達(dá)細(xì)胞系。

pAc5.1 - EGFP是轉(zhuǎn)導(dǎo)生物公司自行改造的昆蟲(chóng)細(xì)胞表達(dá)質(zhì)粒，用于表達(dá)含EGFP(Enhanced Green Fluorescent Protein,增強(qiáng)綠色熒光蛋白)標(biāo)簽的融合蛋白。該質(zhì)粒含有Ac5（果蠅actin 5）啟動(dòng)子，可以高效啟動(dòng)目的蛋白在細(xì)胞中的表達(dá)。在多克隆位點(diǎn)區(qū)有一個(gè)EGFP的完整編碼序列，因此在多克隆位點(diǎn)根據(jù)閱讀框插入目的基因就可以表達(dá)C端或N端含有EGFP標(biāo)簽的融合蛋白。利用EGFP的熒光特性可以比較容易地觀察融合蛋白的表達(dá)水平和細(xì)胞內(nèi)定位，也可以利用EGFP抗體來(lái)檢測(cè)或免疫沉淀融合蛋白。

載體序列

ORIGIN

        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga

       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac

      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg

      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga

      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg

      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc

      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac

      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct

      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag

      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt

      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac

      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt

      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg

      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct

      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca

      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg

      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg

     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc

     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg

     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa

     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa

     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag

     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa

     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt

     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag

     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta

     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga

     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc

     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc

     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca

     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat

     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta

     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag

     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta

     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac

     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa

     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga

     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca

     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa

     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact

     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg

     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct

     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg

     2581 tacctactag tccagtgtgg tggaattctg cagatatcca gcacagtggc ggccgctcga

     2641 gtctagaggg cccgcggttc gaaatcaaaa tggtgagcaa gggcgaggag ctgttcaccg

     2701 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt

     2761 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca

     2821 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt

     2881 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg

     2941 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg

     3001 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact

     3061 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg

     3121 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca

     3181 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg

     3241 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag

     3301 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca

     3361 ctctcggcat ggacgagctg tacaagtaag tttaaacccg ctgatcagcc tcgactgtgc

     3421 cttctaaggc ctgagctcgc tgatcagcct cgatcgagga tccagacatg ataagataca

     3481 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa

     3541 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca

     3601 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca

     3661 agtaaaacct ctacaaatgt ggtatggctg attatgatca gtcgaccgat gcccttgaga

     3721 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt

     3781 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt

     3841 ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc

     3901 ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc

     3961 gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg

     4021 ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc

     4081 gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag

     4141 cttcaaggat cgctcgcggc tcttaccagc cagcaaaagg ccaggaaccg taaaaaggcc

     4201 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc

     4261 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga

     4321 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt

     4381 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg

     4441 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc

     4501 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg

     4561 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc

     4621 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg

     4681 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc

     4741 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct

     4801 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt

     4861 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa

     4921 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa

     4981 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc

     5041 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct

     5101 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca

     5161 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt

     5221 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt

     5281 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc

     5341 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc

     5401 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt

     5461 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact

     5521 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc

     5581 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt

     5641 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg

     5701 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct

     5761 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa

     5821 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt

     5881 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc

     5941 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc

     6001 tataaaaata ggcgtatcac gaggcccttt cgt

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