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基本信息

質(zhì)粒圖譜和多克隆位點信息 

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載體描述

The vector pAc5.1/V5-His offers the strong, constitutive promoter from the Drosophila actin 5C gene. The pAc5.1/V5-His vector also offers the following features:

 Small size (5.4 kb) to improve DNA yields and increase subcloning efficiency

 C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody

 C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin

To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the coding sequence of the C-terminal tag.

載體序列

ORIGIN

        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga

       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac

      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg

      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga

      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg

      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc

      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac

      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct

      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag

      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt

      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac

      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt

      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg

      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct

      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca

      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg

      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg

     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc

     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg

     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa

     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa

     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag

     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa

     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt

     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag

     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta

     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga

     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc

     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc

     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca

     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat

     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta

     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag

     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta

     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac

     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa

     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga

     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca

     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa

     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact

     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg

     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct

     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg

     2581 tacctactag tccagtgtgg tggaattctg cagatatcca gcacagtggc ggccgctcga

     2641 gtctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt

     2701 ctacgcgtac cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga

     2761 ctgtgccttc taaggcctga gctcgctgat cagcctcgat cgaggatcca gacatgataa

     2821 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt

     2881 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta

     2941 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt

     3001 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcagtcg accgatgccc

     3061 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc

     3121 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg

     3181 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg

     3241 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt

     3301 ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg

     3361 ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc

     3421 ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag

     3481 ggacagcttc aaggatcgct cgcggctctt accagccagc aaaaggccag gaaccgtaaa

     3541 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat

     3601 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc

     3661 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc

     3721 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt

     3781 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac

     3841 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg

     3901 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca

     3961 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc

     4021 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa

     4081 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa

     4141 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac

     4201 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta

     4261 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt

     4321 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata

     4381 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc

     4441 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac

     4501 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag

     4561 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac

     4621 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc

     4681 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg

     4741 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc

     4801 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct

     4861 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc

     4921 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc

     4981 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc

     5041 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc

     5101 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca

     5161 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt

     5221 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt

     5281 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca

     5341 ttaacctata aaaataggcg tatcacgagg ccctttcgt

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