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基本信息

體類型: 

大腸桿菌表達(dá)載體 

載體抗性: 

Amp 

載體標(biāo)簽: 

N-His 

啟動(dòng)子 

T7 

篩選標(biāo)記:

/ 

表達(dá)水平: 

高拷貝 

載體大小: 

5711bp 

克隆方法: 

多克隆位點(diǎn),限制性內(nèi)切酶 

5' 測序引物: 

T75-TAATACGACTCACTATAGGG-3 

3' 測序引物 

T7-ter5-GCTAGTTATTGCTCAGCGG-3 

備注:

如有侵權(quán)請(qǐng)產(chǎn)權(quán)持有人盡快聯(lián)系法務(wù)15927229196

質(zhì)粒圖譜和多克隆位點(diǎn)信息

注意:

客戶在我公司選擇構(gòu)建載體服務(wù)項(xiàng)目時(shí),請(qǐng)自行提供母源載體。

如要使用本公司保存的母源載體時(shí),本公司不能保證所有母源載體完全符合客戶預(yù)期,也不承擔(dān)由此產(chǎn)生的任何責(zé)任。

  

載體序列

        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa

       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg

      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt

      181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag

      241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt

      301 cgggctttgt tagcagccgg atcctcgagc atatgacgac cttcgatatg gccgctgctg

      361 tgatgatgat gatgatgatg atgatgatgg cccatggtat atctccttct taaagttaaa

      421 caaaattatt tctagagggg aattgttatc cgctcacaat tcccctatag tgagtcgtat

      481 taatttcgcg ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc

      541 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg

      601 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg

      661 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc

      721 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt

      781 cgagatcccg gacaccatcg aatggcgcaa aacctttcgc ggtatggcat gatagcgccc

      841 ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga

      901 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc

      961 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg

     1021 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct

     1081 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg

     1141 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt

     1201 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca

     1261 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc

     1321 tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt

     1381 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc

     1441 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca

     1501 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca

     1561 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct

     1621 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt

     1681 gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca

     1741 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca

     1801 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc

     1861 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg

     1921 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgta agttagctca

     1981 ctcattaggc accgggatct cgaccgatgc ccttgagagc cttcaaccca gtcagctcct

     2041 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc

     2101 aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct

     2161 ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc

     2221 aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg

     2281 gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg

     2341 ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca

     2401 tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc

     2461 ttaccagcct aacttcgatc actggaccgc tgatcgtcac ggcgatttat gccgcctcgg

     2521 cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc

     2581 ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca

     2641 cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt

     2701 gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct ccagcagccg

     2761 cacgcggcgc atctcgggca gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct

     2821 gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc

     2881 gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac

     2941 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg

     3001 caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac

     3061 atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat

     3121 ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt

     3181 aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa

     3241 tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg

     3301 ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga

     3361 acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct

     3421 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac

     3481 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt

     3541 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg

     3601 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatatg cggtgtgaaa

     3661 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca

     3721 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg

     3781 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc

     3841 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc

     3901 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac

     3961 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc

     4021 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata

     4081 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc

     4141 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca

     4201 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag

     4261 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta

     4321 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg

     4381 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc

     4441 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt

     4501 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa

     4561 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat

     4621 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga

     4681 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac

     4741 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg

     4801 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg

     4861 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt

     4921 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct

     4981 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat

     5041 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta

     5101 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca

     5161 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat

     5221 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac

     5281 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa

     5341 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt

     5401 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg

     5461 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat

     5521 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt

     5581 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct

     5641 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc

     5701 gtcttcaaga a

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