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基本信息
載體類(lèi)型: | 釀酒酵母表達(dá)載體 |
載體抗性: | Amp |
篩選標(biāo)記: | URA3 |
啟動(dòng)子: | GPD |
高拷貝/低拷貝: | 低拷貝 |
載體大小: | 5778 bp |
克隆方法: | 限制性?xún)?nèi)切酶 |
5' 測(cè)序引物: | GPDpro-F: CGGTAGGTATTGATTGTAATTCTG |
3' 測(cè)序引物: | CYC1ter: GGTTGTCTAACTCCTTCCTT |
備注: | 如有侵權(quán)請(qǐng)產(chǎn)權(quán)持有人盡快聯(lián)系法務(wù)15927229196 |
質(zhì)粒圖譜和多克隆位點(diǎn)信息
注意:
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載體序列
ORIGIN
1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact
2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg
2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
2761 aaccctcact aaagggaaca aaagctggag ctcagtttat cattatcaat actcgccatt
2821 tcaaagaata cgtaaataat taatagtagt gattttccta actttattta gtcaaaaaat
2881 tagcctttta attctgctgt aacccgtaca tgcccaaaat agggggcggg ttacacagaa
2941 tatataacat cgtaggtgtc tgggtgaaca gtttattcct ggcatccact aaatataatg
3001 gagcccgctt tttaagctgg catccagaaa aaaaaagaat cccagcacca aaatattgtt
3061 ttcttcacca accatcagtt cataggtcca ttctcttagc gcaactacag agaacagggg
3121 cacaaacagg caaaaaacgg gcacaacctc aatggagtga tgcaacctgc ctggagtaaa
3181 tgatgacaca aggcaattga cccacgcatg tatctatctc attttcttac accttctatt
3241 accttctgct ctctctgatt tggaaaaagc tgaaaaaaaa ggttgaaacc agttccctga
3301 aattattccc ctacttgact aataagtata taaagacggt aggtattgat tgtaattctg
3361 taaatctatt tcttaaactt cttaaattct acttttatag ttagtctttt ttttagtttt
3421 aaaacaccag aacttagttt cgacggattc tagaactagt ggatcccccg ggctgcagga
3481 attcgatatc aagcttatcg ataccgtcga cctcgagtca tgtaattagt tatgtcacgc
3541 ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct
3601 gaagtctagg tccctattta tttttttata gttatgttag tattaagaac gttatttata
3661 tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa
3721 accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcggccg gtacccaatt
3781 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
3841 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
3901 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
3961 gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
4021 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
4081 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
4141 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
4201 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
4261 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
4321 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
4381 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttcc tgatgcggta
4441 ttttctcctt acgcatctgt gcggtatttc acaccgcata gggtaataac tgatataatt
4501 aaattgaagc tctaatttgt gagtttagta tacatgcatt tacttataat acagtttttt
4561 agttttgctg gccgcatctt ctcaaatatg cttcccagcc tgcttttctg taacgttcac
4621 cctctacctt agcatccctt ccctttgcaa atagtcctct tccaacaata ataatgtcag
4681 atcctgtaga gaccacatca tccacggttc tatactgttg acccaatgcg tctcccttgt
4741 catctaaacc cacaccgggt gtcataatca accaatcgta accttcatct cttccaccca
4801 tgtctctttg agcaataaag ccgataacaa aatctttgtc gctcttcgca atgtcaacag
4861 tacccttagt atattctcca gtagataggg agcccttgca tgacaattct gctaacatca
4921 aaaggcctct aggttccttt gttacttctt ctgccgcctg cttcaaaccg ctaacaatac
4981 ctgggcccac cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt ctgtatacac
5041 ccgcagagta ctgcaatttg actgtattac caatgtcagc aaattttctg tcttcgaaga
5101 gtaaaaaatt gtacttggcg gataatgcct ttagcggctt aactgtgccc tccatggaaa
5161 aatcagtcaa gatatccaca tgtgttttta gtaaacaaat tttgggacct aatgcttcaa
5221 ctaactccag taattccttg gtggtacgaa catccaatga agcacacaag tttgtttgct
5281 tttcgtgcat gatattaaat agcttggcag caacaggact aggatgagta gcagcacgtt
5341 ccttatatgt agctttcgac atgatttatc ttcgtttcct gcaggttttt gttctgtgca
5401 gttgggttaa gaatactggg caatttcatg tttcttcaac actacatatg cgtatatata
5461 ccaatctaag tctgtgctcc ttccttcgtt cttccttctg ttcggagatt accgaatcaa
5521 aaaaatttca aagaaaccga aatcaaaaaa aagaataaaa aaaaaatgat gaattgaatt
5581 gaaaagctgt ggtatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
5641 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
5701 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
5761 catcaccgaa acgcgcga
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