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基本信息
載體類型: | 植物表達(dá)載體 |
載體抗性: | Kan |
載體標(biāo)簽: | C-EGFP |
篩選標(biāo)記: | 潮霉素 |
啟動子: | CaMV35s |
表達(dá)水平: | 高拷貝 |
載體大小: | 11159bp |
克隆方法: | 多克隆位點(diǎn),限制性內(nèi)切酶 |
5' 測序引物: | / |
3' 測序引物: | / |
備注: | 如有侵權(quán)請產(chǎn)權(quán)持有人盡快聯(lián)系法務(wù)15927229196 |
質(zhì)粒圖譜和多克隆位點(diǎn)信息
注意:
客戶在我公司選擇構(gòu)建載體服務(wù)項(xiàng)目時,請自行提供母源載體。
如要使用本公司保存的母源載體時,本公司不能保證所有母源載體完全符合客戶預(yù)期,也不承擔(dān)由此產(chǎn)生的任何責(zé)任。
載體描述
在pCAMBIA1300-35S的基礎(chǔ)上,加入EGFP蛋白序列,便于定位和檢測。
載體序列
ORIGIN
1 gaattcggtc cccagattag ccttttcaat ttcagaaaga atgctaaccc acagatggtt
61 agagaggctt acgcagcagg tctcatcaag acgatctacc cgagcaataa tctccaggaa
121 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc
181 aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt
241 caaggcttgc ttcacaaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt
301 cccactgaat caaaggccat ggagtcaaag attcaaatag aggacctaac agaactcgcc
361 gtaaagactg gcgaacagtt catacagagt ctcttacgac tcaatgacaa gaagaaaatc
421 ttcgtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc
481 tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc
541 ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc
601 tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac
661 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
721 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
781 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
841 agaacacggg ggacgagctc ggtacccggg gatcctctag agtcgacatg gtgagcaagg
901 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
961 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
1021 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
1081 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
1141 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
1201 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
1261 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
1321 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
1381 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
1441 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
1501 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
1561 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaactg cagagctttc
1621 gttcgtatca tcggtttcga caacgttcgt caagttcaat gcatcagttt cattgcgcac
1681 acaccagaat cctactgagt tcgagtatta tggcattggg aaacatgttt ttcttgtacc
1741 atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa
1801 tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt ctcaaattaa
1861 tattatttgt tttttctctt atttgttgtg tgttgaattt gaaaatataa gagatatgca
1921 aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg aagttaatat
1981 gaggagtaaa acacttgtag ttgtaccatt atgcttattc actaggcaac aaatatattt
2041 tcagacctag aaaagctgca aatgttactg aatacaagta tgtcctcttg tgttttagac
2101 atttatgaac tttcctttat gtaattttcc agaatccttg tcagattcta atcattgctt
2161 tataattata gttatactca tggatttgta gttgagtatg aaaatatttt ttaatgcatt
2221 ttatgacttg ccaattgatt gacaacatgc atcaagcttg gcactggccg tcgttttaca
2281 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
2341 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
2401 cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg tttcccgcct
2461 tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag
2521 cgtttattag aataacggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt
2581 gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc
2641 cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg
2701 tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt
2761 gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc
2821 atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag
2881 gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag
2941 aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc
3001 cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta
3061 ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg
3121 tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc
3181 gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc
3241 cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc
3301 gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat
3361 cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg
3421 cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag
3481 aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt
3541 tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg
3601 cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg
3661 cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat
3721 gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa
3781 ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg
3841 gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga
3901 tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga
3961 agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc
4021 catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt
4081 gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat
4141 atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag
4201 gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt
4261 tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt
4321 gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga
4381 cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa
4441 tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca
4501 cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc
4561 aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc
4621 aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa
4681 tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca
4741 accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt
4801 aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc
4861 ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
4921 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
4981 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
5041 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
5101 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
5161 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
5221 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
5281 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
5341 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
5401 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
5461 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
5521 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
5581 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg
5641 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
5701 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
5761 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
5821 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
5881 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
5941 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc
6001 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
6061 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
6121 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
6181 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
6241 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
6301 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
6361 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat
6421 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
6481 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
6541 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
6601 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
6661 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
6721 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
6781 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
6841 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
6901 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
6961 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
7021 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
7081 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
7141 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
7201 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
7261 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
7321 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
7381 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
7441 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
7501 aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat tcatccagta
7561 aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct
7621 cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat
7681 accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt
7741 tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct
7801 tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc
7861 agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc
7921 ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga
7981 tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg
8041 agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa
8101 agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc
8161 gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt
8221 cattttctcc caccagctta tataccttag caggagacat tccttccgta tcttttacgc
8281 agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt
8341 atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac
8401 gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc
8461 aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg
8521 gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat
8581 catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg
8641 agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg
8701 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt
8761 ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg
8821 acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat tttagtactg
8881 gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa tacaaataca
8941 tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac cctaattccc
9001 ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga tcgacagatc
9061 cggtcggcat ctactctatt tctttgccct cggacgagtg ctggggcgtc ggtttccact
9121 atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg
9181 tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca
9241 agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg
9301 gagcatatac gcccggagtc gtggcgatcc tgcaagctcc ggatgcctcc gctcgaagta
9361 gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc
9421 gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt
9481 gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca
9541 gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc
9601 gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg
9661 ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct
9721 aagatcggcc gcagcgatcg catccatagc ctccgcgacc ggttgtagaa cagcgggcag
9781 ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga tgcaataggt
9841 caggctctcg ctaaactccc caatgtcaag cacttccgga atcgggagcg cggccgatgc
9901 aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag
9961 gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag
10021 ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc
10081 ggtgagttca ggctttttca tatctcattg ccccccggga tctgcgaaag ctcgagagag
10141 atagatttgt agagagagac tggtgatttc agcgtgtcct ctccaaatga aatgaacttc
10201 cttatataga ggaaggtctt gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt
10261 ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac
10321 gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac
10381 gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt
10441 ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac
10501 cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt
10561 ggagtagacg agagtgtcgt gctccaccat gttatcacat caatccactt gctttgaaga
10621 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
10681 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt
10741 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg
10801 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc
10861 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt
10921 ggcaagctgc tctagccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
10981 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
11041 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
11101 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattac
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