您所在的位置:首頁(yè) > 載體庫(kù) > 真菌相關(guān)載體
pGBKT7-lam 構(gòu)建費(fèi)用300元/個(gè)起

請(qǐng)?jiān)诰€咨詢(xún)或直接聯(lián)系18986294316/18171096062

SMART 快構(gòu) 請(qǐng)注冊(cè)登錄 http://49.234.53.21/login

基本信息

載體類(lèi)型: 

酵母單雜交載體 

載體抗性: 

Kan 

篩選標(biāo)記: 

TRP1 

啟動(dòng)子: 

ADH1 

載體大小: 

7.9kb 

5' 測(cè)序引物: 

T7:TAATACGACTCACTATAGGG 

3' 測(cè)序引物: 

根據(jù)序列設(shè)計(jì)引物 

備注

如有侵權(quán)請(qǐng)產(chǎn)權(quán)持有人盡快聯(lián)系法務(wù)15927229196

質(zhì)粒圖譜和多克隆位點(diǎn)信息

注意:

客戶(hù)在我公司選擇構(gòu)建載體服務(wù)項(xiàng)目時(shí),請(qǐng)自行提供母源載體。

如要使用本公司保存的母源載體時(shí),本公司不能保證所有母源載體完全符合客戶(hù)預(yù)期,也不承擔(dān)由此產(chǎn)生的任何責(zé)任。


載體描述

pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein (a.a. 66–230) and the GAL4 DNA-BD (a.a. 1–147). The lamin C cDNA insert (GenBank Accession #M13451) was derived from the plasmid referenced in Bartel et al. (1993a). Plasmid modification was performed atBD Biosciences Clontech. Yeast cotransformed with pGBKT7-Lam and pGADT7-RecT, provide a measure of the backgroundthat is due to false-positive two-hybrid interactions. pGBKT7-Lam has not been sequenced.