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基本信息

質(zhì)粒圖譜和多克隆位點(diǎn)信息

注意：

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由pTOL2-MCS改造而來(lái)，加入mCherry標(biāo)簽，可用于啟動(dòng)子活性檢測(cè)。

載體序列

ORIGIN

        1 agaaccagag gtgtaaagta cttgagtaat tttacttgat tactgtactt aagtattatt

       61 tttggggatt tttactttac ttgagtacaa ttaaaaatca atacttttac ttttacttaa

      121 ttacattttt ttagaaaaaa aagtactttt tactccttac aattttattt acagtcaaaa

      181 agtacttatt ttttggagat cacttagatc taactgcaga acgtacgaac ccgggaaacg

      241 cgttctagag tcgacggtac cggatccggg cccgccacca tggtgagcaa gggcgaggag

      301 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg

      361 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag

      421 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc

      481 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac

      541 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc

      601 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg

      661 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc

      721 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag

      781 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag

      841 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc

      901 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc

      961 accggcggca tggacgagct gtacaagtag ctcgacaact tgtttattgc agcttataat

     1021 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat

     1081 tctagttgtg gtttgtccaa actcatcaat gtatcttact cgagaaaagc ttaaacaaga

     1141 atctctagtt ttctttcttg cttttacttt tacttcctta atactcaagt acaattttaa

     1201 tggagtactt ttttactttt actcaagtaa gattctagcc agatactttt acttttaatt

     1261 gagtaaaatt ttccctaagt acttgtactt tcacttgagt aaaatttttg agtacttttt

     1321 acacctctga attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc

     1381 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga

     1441 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg

     1501 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg

     1561 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg

     1621 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga

     1681 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg

     1741 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag

     1801 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc

     1861 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg

     1921 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt

     1981 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc

     2041 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc

     2101 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg

     2161 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca

     2221 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc

     2281 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat

     2341 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt

     2401 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt

     2461 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc

     2521 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc

     2581 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata

     2641 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg

     2701 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc

     2761 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct

     2821 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa

     2881 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt

     2941 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca

     3001 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac

     3061 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca

     3121 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt

     3181 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc

     3241 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca

     3301 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata

     3361 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc

     3421 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc

     3481 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat

     3541 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga

     3601 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa

     3661 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca

     3721 tcagagcaga ttgtactgag agtgcaccat atg

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