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基本信息

載體類型： 

釀酒酵母蛋白表達載體 

載體抗性： 

Amp

載體標簽： 

C-Flag, C-Myc 

篩選標記： 

His3 

啟動子： 

GAL1和GAL10 

表達水平: 

高拷貝 

載體大小: 

6631 bp 

克隆方法: 

多克隆位點，限制性內切酶 

5' 測序引物: 

GAL1-F: 5’-ATTTTCGGTTTGTATTACTTC-3’; 

GAL10-F: 5’-GGTGGTAATGCCATGTAATATG-3’ 

3' 測序引物： 

GALI-R: 5’-GTTCTTAATACTAACATAACT-3’ 

GAL10-R: 5’-GGCAAGGTAGACAAGCCGACAAC-3’ 

備注：

如有侵權請產(chǎn)權持有人盡快聯(lián)系法務15927229196

質粒圖譜和多克隆位點信息

注意：

客戶在我公司選擇構建載體服務項目時，請自行提供母源載體。

如要使用本公司保存的母源載體時，本公司不能保證所有母源載體完全符合客戶預期，也不承擔由此產(chǎn)生的任何責任。

載體描述

帶有URA3標記的酵母表達載體，用半乳糖調節(jié)表達和標記多達2個基因。

載體序列

ORIGIN

        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca

       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg

      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc

      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc

      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg

      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc

      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt

      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat

      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca

      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg

      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg

      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca

      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac

      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa

      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg

      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct

      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac

     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg

     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa

     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa

     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac

     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac

     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat

     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa

     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca

     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg

     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta

     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg

     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa

     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg

     1801 gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc

     1861 ttcgctatta cgccagctga attggagcga cctcatgcta tacctgagaa agcaacctga

     1921 cctacaggaa agagttactc aagaataaga attttcgttt taaaacctaa gagtcacttt

     1981 aaaatttgta tacacttatt ttttttataa cttatttaat aataaaaatc ataaatcata

     2041 agaaattcgc ttatttagaa gtgtcaacaa cgtatctacc aacgatttga cccttttcca

     2101 tcttttcgta aatttctggc aaggtagaca agccgacaac cttgattgga gacttgacca

     2161 aacctctggc gaagaattgt taattaagag ctcagatctt atcgtcgtca tccttgtaat

     2221 ccatcgatac tagtgcggcc gccctttagt gagggttgaa ttcgaatttt caaaaattct

     2281 tacttttttt ttggatggac gcaaagaagt ttaataatca tattacatgg cattaccacc

     2341 atatacatat ccatatacat atccatatct aatcttactt atatgttgtg gaaatgtaaa

     2401 gagccccatt atcttagcct aaaaaaacct tctctttgga actttcagta atacgcttaa

     2461 ctgctcattg ctatattgaa gtacggatta gaagccgccg agcgggtgac agccctccga

     2521 aggaagactc tcctccgtgc gtcctcgtct tcaccggtcg cgttcctgaa acgcagatgt

     2581 gcctcgcgcc gcactgctcc gaacaataaa gattctacaa tactagcttt tatggttatg

     2641 aagaggaaaa attggcagta acctggcccc acaaaccttc aaatgaacga atcaaattaa

     2701 caaccatagg atgataatgc gattagtttt ttagccttat ttctggggta attaatcagc

     2761 gaagcgatga tttttgatct attaacagat atataaatgc aaaaactgca taaccacttt

     2821 aactaatact ttcaacattt tcggtttgta ttacttctta ttcaaatgta ataaaagtat

     2881 caacaaaaaa ttgttaatat acctctatac tttaacgtca aggagaaaaa accccggatc

     2941 cgtaatacga ctcactatag ggcccgggcg tcgacatgga acagaagttg atttccgaag

     3001 aagacctcga gtaagcttgg taccgcggct agctaagatc cgctctaacc gaaaaggaag

     3061 gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg ttagtattaa

     3121 gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg tacgcatgta

     3181 acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaagatc cagctgcatt

     3241 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct

     3301 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa

     3361 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa

     3421 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc

     3481 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga

     3541 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc

     3601 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt

     3661 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct

     3721 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg

     3781 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta

     3841 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct

     3901 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa

     3961 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt

     4021 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta

     4081 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat

     4141 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa

     4201 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct

     4261 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta

     4321 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct

     4381 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg

     4441 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa

     4501 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt

     4561 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta

     4621 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca

     4681 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta

     4741 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct

     4801 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg

     4861 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac

     4921 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact

     4981 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa

     5041 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt

     5101 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat

     5161 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg

     5221 aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt

     5281 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt

     5341 taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa

     5401 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc

     5461 tattttacca acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc

     5521 gctatttttc taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg

     5581 cagtctcttg ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg

     5641 tgtctatttt ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta

     5701 gcgaagctgc gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat

     5761 gtggattgcg catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag

     5821 aaaattatga acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca

     5881 ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa

     5941 gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg

     6001 agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga

     6061 tacttttgag caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc

     6121 cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc

     6181 tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaactt caaagcgttt

     6241 ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc acatacagct cactgttcac

     6301 gtcgcaccta tatctgcgtg ttgcctgtat atatatatac atgagaagaa cggcatagtg

     6361 cgtgtttatg cttaaatgcg tacttatatg cgtctattta tgtaggatga aaggtagtct

     6421 agtacctcct gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta

     6481 ccctttagct gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct

     6541 atcatttcct ttgatattgg atcatactaa gaaaccatta ttatcatgac attaacctat

     6601 aaaaataggc gtatcacgag gccctttcgt c

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