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pCDNA3.1(+) 構(gòu)建費用200元/個起

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基本信息

載體類型: 

哺乳動物細(xì)胞表達載體 

載體抗性: 

Amp 

篩選標(biāo)記: 

Neomycin / G418 

啟動子: 

CMV 

載體大小: 

5428bp 

克隆方法: 

多克隆位點,限制性內(nèi)切酶 

5' 測序引物: 

/ 

3' 測序引物: 

/ 

備注:

如有侵權(quán)請產(chǎn)權(quán)持有人盡快聯(lián)系法務(wù)15927229196

質(zhì)粒圖譜和多克隆位點信息

注意:

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如要使用本公司保存的母源載體時,本公司不能保證所有母源載體完全符合客戶預(yù)期,也不承擔(dān)由此產(chǎn)生的任何責(zé)任。



載體序列

ORIGIN

        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg

       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg

      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc

      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt

      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata

      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc

      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc

      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt

      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt

      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca

      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg

      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc

      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg

      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca

      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc

      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc

      961 agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca

     1021 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc

     1081 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg

     1141 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg

     1201 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag

     1261 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta

     1321 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg

     1381 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa

     1441 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc

     1501 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt

     1561 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca

     1621 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc

     1681 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg

     1741 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca

     1801 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa

     1861 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca

     1921 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt

     1981 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag

     2041 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg

     2101 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg

     2161 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa

     2221 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg

     2281 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt

     2341 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa

     2401 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc

     2461 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg

     2521 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg

     2581 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg

     2641 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg

     2701 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact

     2761 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg

     2821 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc

     2881 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct

     2941 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac

     3001 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat

     3061 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc

     3121 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc

     3181 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc

     3241 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg

     3301 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg

     3361 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc

     3421 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt

     3481 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct

     3541 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga

     3601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc

     3661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg

     3721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg

     3781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt

     3841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt

     3901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg

     3961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact

     4021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt

     4081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct

     4141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac

     4201 cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca

     4261 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta

     4321 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa

     4381 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg

     4441 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg

     4501 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc

     4561 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc

     4621 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa

     4681 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc

     4741 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg

     4801 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc

     4861 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat

     4921 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg

     4981 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc

     5041 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg

     5101 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat

     5161 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg

     5221 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg

     5281 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct

     5341 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac

     5401 atttccccga aaagtgccac ctgacgtc

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