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基本信息

質(zhì)粒圖譜和多克隆位點(diǎn)信息

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載體描述

pcDNA3.1(+) and pcDNA3.1(-) are 5.4 kb vectors derived from pcDNA3 and designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements: ? Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells ? Multiple cloning sites in the forward (+) and reverse (-) orientations to facilitate cloning ? Neomycin resistance gene for selection of stable cell lines ? Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7). The control plasmid, pcDNA3.1/CAT, is included for use as a positive control for transfection and expression in the cell line of choice.

載體序列

ORIGIN

        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg

       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg

      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc

      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt

      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata

      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc

      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc

      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt

      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt

      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca

      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg

      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc

      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg

      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca

      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc

      901 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcc

      961 accacactgg actagtggat ccgagctcgg taccaagctt aagtttaaac cgctgatcag

     1021 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct

     1081 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc

     1141 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg

     1201 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg

     1261 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa

     1321 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc

     1381 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag

     1441 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca

     1501 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc

     1561 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa

     1621 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct

     1681 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt

     1741 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat

     1801 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag

     1861 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat

     1921 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt

     1981 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg

     2041 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg

     2101 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc

     2161 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat

     2221 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt

     2281 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg

     2341 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag

     2401 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc

     2461 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc

     2521 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga

     2581 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga

     2641 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg

     2701 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg

     2761 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc

     2821 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc

     2881 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg

     2941 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc

     3001 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc

     3061 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct

     3121 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca

     3181 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg

     3241 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt

     3301 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt

     3361 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg

     3421 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg

     3481 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg

     3541 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat

     3601 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc

     3661 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc

     3721 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga

     3781 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt

     3841 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg

     3901 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc

     3961 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg

     4021 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc

     4081 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg

     4141 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc

     4201 gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa

     4261 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa

     4321 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa

     4381 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc

     4441 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga

     4501 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca

     4561 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc

     4621 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat

     4681 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc

     4741 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt

     4801 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc

     4861 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg

     4921 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt

     4981 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg

     5041 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga

     5101 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg

     5161 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg

     5221 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt

     5281 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc

     5341 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca

     5401 tttccccgaa aagtgccacc tgacgtc

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