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基本信息
載體類型: | 哺乳動物細胞表達載體;紅色熒光報告載體 |
載體抗性: | Kan |
載體標簽: | DsRed-Express,紅色熒光蛋白 |
篩選標記: | 新霉素 |
啟動子: | CMV |
表達水平: | 高拷貝 |
載體大小: | 4691bp |
克隆方法: | 多克隆位點,限制性內(nèi)切酶 |
5' 測序引物: | CMV-F: 5’-CGCAAATGGGCGGTAGGCGTG-3’ |
3' 測序引物: | / |
備注: | 如有侵權(quán)請產(chǎn)權(quán)持有人盡快聯(lián)系法務(wù)15927229196 |
質(zhì)粒圖譜和多克隆位點信息
注意:
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載體序列
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
661 gatccaccgg tcgccaccat ggacaacacc gaggacgtca tcaaggagtt catgcagttc
721 aaggtgcgca tggagggctc cgtgaacggc cactacttcg agatcgaggg cgagggcgag
781 ggcaagccct acgagggcac ccagaccgcc aagctgcagg tgaccaaggg cggccccctg
841 cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggc ctacgtgaag
901 caccccgccg acatccccga ctacatgaag ctgtccttcc ccgagggctt cacctgggag
961 cgctccatga acttcgagga cggcggcgtg gtggaggtgc agcaggactc ctccctgcag
1021 gacggcacct tcatctacaa ggtgaagttc aagggcgtga acttccccgc cgacggcccc
1081 gtaatgcaga agaagactgc cggctgggag ccctccaccg agaagctgta cccccaggac
1141 ggcgtgctga agggcgagat ctcccacgcc ctgaagctga aggacggcgg ccactacacc
1201 tgcgacttca agaccgtgta caaggccaag aagcccgtgc agctgcccgg caaccactac
1261 gtggactcca agctggacat caccaaccac aacgaggact acaccgtggt ggagcagtac
1321 gagcacgccg aggcccgcca ctccggctcc cagtagagcg gccgcgactc tagatcataa
1381 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
1441 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
1501 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
1561 attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt
1621 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
1681 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
1741 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
1801 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
1861 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
1921 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
1981 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
2041 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
2101 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
2161 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga
2221 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
2281 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
2341 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
2401 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
2461 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
2521 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg
2581 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
2641 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
2701 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
2761 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
2821 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
2881 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
2941 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
3001 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
3061 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
3121 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
3181 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
3241 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
3301 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
3361 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
3421 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
3481 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
3541 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
3601 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc
3661 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
3721 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
3781 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat
3841 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
3901 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
3961 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
4021 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
4081 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
4141 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
4201 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
4261 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
4321 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
4381 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
4441 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
4501 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
4561 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
4621 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
4681 ccgccatgca t
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