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基本信息

載體類型： 

哺乳動(dòng)物細(xì)胞表達(dá)載體，熒光蛋白報(bào)告載體 

載體抗性： 

Kan

載體標(biāo)簽： 

C-EGFP 

篩選標(biāo)記： 

新霉素/G418 

啟動(dòng)子： 

CMV 

表達(dá)水平: 

高拷貝 

載體大小: 

4729bp 

克隆方法: 

多克隆位點(diǎn)，限制性內(nèi)切酶 

5' 測(cè)序引物: 

CMV-F: 5'-CGCAAATGGGCGGTAGGCGTG-3' 

3' 測(cè)序引物： 

EGFP-N: 5'-CGTCGCCGTCCAGCTCGACCAG-3' 

備注：

如有侵權(quán)請(qǐng)產(chǎn)權(quán)持有人盡快聯(lián)系法務(wù)15927229196

質(zhì)粒圖譜和多克隆位點(diǎn)信息

注意：

客戶在我公司選擇構(gòu)建載體服務(wù)項(xiàng)目時(shí)，請(qǐng)自行提供母源載體。

如要使用本公司保存的母源載體時(shí)，本公司不能保證所有母源載體完全符合客戶預(yù)期，也不承擔(dān)由此產(chǎn)生的任何責(zé)任。


載體序列

ORIGIN

        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg

       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt

      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca

      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc

      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta

      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac

      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg

      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg

      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt

      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta

      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg

      661 gatccatcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc

      721 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg

      781 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg

      841 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc

      901 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg

      961 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg

     1021 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca

     1081 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg

     1141 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca

     1201 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc

     1261 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc

     1321 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg

     1381 agctgtacaa gtaaagcggc cgcgactcta gatcataatc agccatacca catttgtaga

     1441 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa

     1501 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag

     1561 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa

     1621 actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt

     1681 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt

     1741 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc

     1801 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg

     1861 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac

     1921 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg

     1981 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag

     2041 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt

     2101 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac

     2161 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa

     2221 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg

     2281 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc

     2341 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct

     2401 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc

     2461 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga

     2521 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg

     2581 cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg

     2641 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac

     2701 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg

     2761 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc

     2821 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg

     2881 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc

     2941 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc

     3001 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta

     3061 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg

     3121 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg

     3181 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat

     3241 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc

     3301 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta

     3361 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag

     3421 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt

     3481 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg

     3541 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag

     3601 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa

     3661 aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca

     3721 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc

     3781 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt

     3841 cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa

     3901 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa

     3961 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg

     4021 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc

     4081 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac

     4141 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca

     4201 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt

     4261 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc

     4321 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg

     4381 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc

     4441 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac

     4501 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct

     4561 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc

     4621 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt

     4681 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcat

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